Trypan blue, powder, biological stain, 99.96%, Analytical Reagent (ACS)

Description

Trypan blue, powder, biological stain, 99.96%, Analytical Reagent (ACS)
Linear Formula: C34H24N6O14S4Na4,
CAS Number: 72-57-1,
Synonym:
Azidine blue 3B
Benzamine blue 3B
Benzo Blue bB
Chlorazol blue 3B
Diamine blue 3B
Dianil blue H3G
Direct blue 14
Niagara blue 3B
IUPAC name (3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonic acid)
Trypan blue is an azo dye that is used as a dye-stuff. It is a direct dye for cotton textiles. In biosciences, it is used as vital stain to selectively colour dead tissues or cells blue.
Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.
FOR LABORATORY USE ONLY.